How to prevent liquid pump failure

To maintain good pump performance, the system must be kept clean, the quality of the solvents and reagents used must be maintained and the mobile phase must be filtered and degassed before entering the flow path. Preventing pump failure requires a long-lasting effort: 1) Use of high-quality reagents and HPLC-grade solvents will benefit the pump on the column; 2) Mobile phases and solvents are filtered using premium materials prior to use; 3) Mobile phase and solvent Into the process of degassing; 4) each time you start to use the exhaust vent, after work from the pump and column wash buffer or harmful substances; do not allow water and corrosive solvents and other harmful substances stuck in the pump and the column ; 5) Regular replacement of washers, such as online filter rings; and 6) Strict compliance with pump operation manual recommendations. Pumps in good working order should be able to stabilize the baseline on the chromatogram; reproducibility of retention time is good. Fluctuations in isocratic elution are less than 2%. Gradient elution pressure changes should be slow and steady. In order to rule out the failure as soon as possible after the failure, usually should be standing seal, check valve (inlet and outlet), pump head device, all kinds of fittings, fuses and other components, and replacement tools.

Hot Pipette Tips

The commonly used Pipette kits are mainly composed of a pipette and a pipette tip. The pipette tip is a single empty
Cavity reservoir; the pipette uses the principle of air displacement and controls the maximum displacement of the piston by rotating a screw with an ultra-fine thread
displacement, and then control the amount of liquid suction. There are the following problems when using the above kit for suction: 1. The suction volume is controlled by the pipette
After the pipette is sterilized or used for a period of time, it is easy to cause "hidden" failures, which cause the operator to be difficult to detect.
The actual suction volume is inaccurate, and it needs to be adjusted frequently, and the operation is cumbersome and time-consuming; 2. To improve the pipetting accuracy, the operator's
The operation requirements are high, and it is easily affected by factors such as the suction speed, the depth of the tip below the liquid level, and the inclination angle of the pipette.
The actual suction volume will be heard; 3. The actual suction volume will be affected because the suction head cover is not tight and leaking, and it will not be easily detected; 4.
When performing experiments that require high accuracy, such as analysis, in the operation of equal volume aspiration of liquids with different densities, the actual
The actual suction volume will be affected by the density itself; 5. In order to improve the accuracy, this type of pipette needs to carry out high
Cost-optimized design results in extremely high pipette costs.

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